Fully printed one-step biosensing device using graphene/AuNPs composite

Bhawna Nagar, Marc Balsells, Alfredo de la Escosura-Muñiz, Pedro Gomez-Romero, Arben Merkoçi. Biosensors and Bioelectronics, 2019, Vol 129, pp 238-244
Driven by the growing need of simple, cost efficient and flexible sensing systems, we have designed here a fully printed Reduced Graphene Oxide (rGO) based impedimetric sensor for one step sensing of DNA. The DNA sensor was fabricated by stamping of layered rGO and rGO/gold nanoparticles/single stranded DNA (rGO/AuNPs/ssDNA) composites...

Iridium oxide (IV) nanoparticle-based lateral flow immunoassay

Daniel Quesada-González, Amadeo Sena-Torralba, Wiyogo Prio Wicaksono, Alfredo de la Escosura-Muñiz, Tribidasari A. Ivandini, Arben Merkoçi. Biosensors and Bioelectronics, 2019, Vol 132, pp 132-135,
Lateral flow biosensors are paper-based devices that allow the detection of different types of analytes with quickness, robustness, and selectivity, without leaving behind paper sensors benefits as low-cost, recyclability and sustainability. Nanomaterials have been widely reported in lateral flow biosensors, offering new sensing...

Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers

Daniel Quesada-González, Christina Stefani, Israel González, Alfredo de la Escosura-Muñiz, Neus Domingo, Pere Mutjé, Arben Merkoçi. Biosensors and Bioelectronics, 2019, Vol 141, pp 111407
Lateral flow paper-based biosensors merge as powerful tools in point-of-care diagnostics since they are cheap, portable, robust, selective, fast and easy to use. However, the sensitivity of this type of biosensors is not always as high as required, often not permitting a clear quantification. To improve the colorimetric response...

In Situ Plant Virus Nucleic Acid Isothermal Amplification Detection on Gold Nanoparticle-Modified Electrodes

Mohga Khater, Alfredo de la Escosura-Muñiz, Laura Altet, Arben Merkoçi. Analytical Chemistry, 2019, Vol 91 (7), pp 4790-4796
Solid-phase isothermal recombinase polymerase amplification (RPA) offers many benefits over the standard RPA in homogeneous phase in terms of sensitivity, portability, and versatility. However, RPA devices reported to date are limited by the need for heating sources to reach sensitive detection. With the aim of overcoming such limitation,...

Production and printing of graphene oxide foam ink for electrocatalytic applications

Luis Baptista-Pires, Alfredo de la Escosura-Muñiz, Marc Balsells, Julio C. Zuaznabar-Gardona, Arben Merkoçi. Electrochemistry Communications, 2019, Vol 98, pp 6-9
A graphene-based ink printed as a foam-like structure with open pores is reported. The production of the ink is easier and faster than using existing methods and the obtained product is stable in water suspension. Electrocatalytic applications of 3D structured electrodes printed onto plastic substrates were explored. [Link to...

Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water

Abdel-Rahim H. A. Hassan, José Francisco Bergua, Eden Morales-Narváez, Arben Merkoçi. Food Chemistry, 2019, Vol 297, pp 124965
Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for...

Low-Cost Strategy for the Development of a Rapid Electrochemical Assay for Bacteria Detection Based on AuAg Nanoshells

Lorenzo Russo, Juan Leva Bueno, Jose Francisco Bergua, Monica Constantini, Marco Giannetto, Víctor Puntes, Alfredo de la Escosura Muñiz, Arben Merkoçi. ACS Omega, 2018, Vol 3 (12), pp 18849-18856
A low-cost strategy for the simple and rapid detection of bacterial cells in biological matrixes is presented herein. Escherichia coli and Salmonella typhimurium were chosen as model bacteria for the development of an electrochemical assay based on hollow AuAg nanoshells (NSs). By taking advantage of their electrocatalytic properties...

Nanotecnología en los Biosensores de Papel

Daniel Quesada-González, Arben Merkoçi. Sociedad Española de Química Analítica, nº63
Click on the picture, and then, link to the journal: here

Iridium oxide (IV) nanoparticle-based electrocatalytic detection of PBDE

Damiel Quesada-González, Alessandra Baiocco, Andrea A. Martos, Alfredo de la Escosura-Muñiz, Arben Merkoçi. Biosensors and Bioelectronics, 2019, Vol 127, pp 150-154,
Polybrominated diphenyl ethers (PBDEs) are a type of flame retardants which are currently banned in EU and USA due their hazardousness for humans and mammals. However, these compounds were highly used during more than 30 years and still persist in the environment since they are resistant to degradation. Herein we present a biosensor for...

Electrochemical detection of plant virus using gold nanoparticle-modified electrodes

Mohga Khater, Alfredo de la Escosura-Muñiz, Daniel Quesada-González, Arben Merkoçi. Analytica Chimica Acta, 2019, Vol 1046, pp 123-131,
Tristeza is one of the destructive diseases of citrus causing by citrus tristeza virus (CTV). Historically, CTV has been associated with serious outbreaks of quick decline of citrus, therefore CTV monitoring is important aspect for avoiding such re-emerging epidemics, which would threat citrus production through the world. In...